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KMID : 1094020140310010036
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Journal of Veterinary Clinics
2014 Volume.31 No. 1 p.36 ~ p.39
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Identification of Bacteria by Sequence Analysis of 16S rRNA in Testes of Jeju Horses
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Park Yong-Sang
Kim Nam-Young
Han Sang-Hyun
Park Nam-Geon
Ko Moon-Suck
Cho Won-Mo
Chae Hyun-Seok
Cho In-Cheol
Cho Sang-Rae
Woo Jae-Hoon
Kang Tae-Young
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Abstract
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Many bacteria colonized in the horse semen affect quality of the sperm and some may cause infectionin the mare reproductive tract and infertility of susceptible mare. This study was initiated to determine the prevalenceof bacteria in testes of Jeju horses by determining rRNA sequence. The samples were swabed from the testes of nineJeju horses (aged from 8 to 12 months after birth). Bacteria isolated from testes were identified by 16S rDNA sequencing. 1.6-kbp PCR products for 16S rRNA coding region were obtained using the universal primers. The PCR productswere further purified and sequenced. Maximum similar species were found by BLAST search in the GenBank DNAdatabase. BLAST results showed that the sequences were similar to those of Acinetobacter sp (A. schindleri, A. ursingii).,Bacillus cereus, Corynebacterium glutamicum, Escherichia coli, Gamma proteobacterium, Micrococcus luteus,Pseudomonas mendocina, Shigella sonnei, Sphingomonas sp., Staphylococcus sp (S. cohnii, S. saprophyticus, S. xylosus)., and Stenotrophomonas maltophilia. DNA sequences for 16S rRNA is provided useful informations for speciesidentification of pathogenic microorganisms for the reproductive organs in horses.
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KEYWORD
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Bacterial contamination, Jeju horse, 16S rRNA sequencing
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